Phenolics bind to proteins and DNA through hydrogen bonds immediately after plant cells are lysed and effect their properties and successful extraction, therefore it is important to use phenolic inhibitors.
Polyvinylpyrrolidone (PVP), also commonly called polyvidone or povidone, is a water-soluble polymer made from the monomer N-vinylpyrrolidone that binds polyphenols. PVP is available in a variety of molecular weights. with the smaller molecular weights capable of binding to proteins. For use with proteins, it is recommended to start with 2-4% 40KD PVP. Depending on the specific phenolics in the plant tissue, the molecular weight of PVP will need to be optimized. Different molecular weights bind certain phenolics better than others, so PVP does not bind all phenolics universally.
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